A new Na+/H+ antiporter, NhaD, of Vibrio parahaemolyticus
نویسندگان
چکیده
منابع مشابه
Functional analysis of conserved polar residues in Vc-NhaD, Na+/H+ antiporter of Vibrio cholerae.
Vc-NhaD is a Na(+)/H(+) antiporter from Vibrio cholerae with a sharp maximum of activity at pH approximately 8.0. NhaD homologues are present in many bacteria as well as in higher plants. However, very little is known about structure-function relations in NhaD-type antiporters. In this work 14 conserved polar residues associated with putative transmembrane segments of Vc-NhaD have been screened...
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The activity of the NhaA Na+/H+ antiporter of Vibrio parahaemolyticus is inhibited by amiloride. We found an amino acid sequence in the NhaA that was identical to a putative amiloride binding domain of the Na+/H+ exchanger in mammalian cells. We constructed mutant NhaAs that had amino acid substitutions in the putative amiloride binding domain by site-directed mutagenesis. These include V62L (V...
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The Vc-NhaD is an Na + /H + antiporter from Vibrio cholerae belonging to a new family of bacterial Na + /H + antiporters, the NhaD family. In the present work we mutagenized five conserved Asp and Glu residues and one conserved Thr residue to Ala in order to identify amino acids that are critical for the antiport activity. All mutations fall into two distinct groups: (i) four variants, GluAla, ...
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G. Balakrish Nair es Director del Laboratory Sciences Division, en el International Center for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B)-Centre for Health and Population Research, Dhaka, Bangladesh. Se graduó de Madras University en 1975, realizó una maestría en Biología Marina en 1977 en Annamalai University alcanzando el grado de PhD en 1982. Entonces se integró al Department of Micr...
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Movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. However, not all V. parahaemolyticus isolates swarm proficiently. The organism undergoes phase variation between opaque (OP) and transluce...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 1998
ISSN: 0005-2736
DOI: 10.1016/s0005-2736(97)00223-x